mate plate human bone marrow cdna library (TaKaRa)
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TaKaRa
mate plate human bone marrow cdna library
Mate Plate Human Bone Marrow Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mate+plate+human+bone+marrow+cdna+library/Human+Bone+Marrow+QUICK-Clone+cDNA/pm37129527-245-27-35
Average 94 stars, based on 212 article reviews
Mate Plate Human Bone Marrow Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mate+plate+human+bone+marrow+cdna+library/Human+Bone+Marrow+QUICK-Clone+cDNA/pm37129527-245-27-35
Average 94 stars, based on 212 article reviews
mate plate human bone marrow cdna library - by Bioz Stars,
2026-09
94/100 stars
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other:Article Title: A Brucella effector modulates the Arf6-Rab8a GTPase cascade to promote intravacuolar replication. Article Snippet: Y187 (pGADT7) mated with either Y2HGold (pGBKT7 vector) or Y2HGold (pGBKT7bspF) acted as the negative controls. cDNA Library Assay:Article Title: A Brucella effector modulates the Arf6‐Rab8a GTPase cascade to promote intravacuolar replication Article Snippet: The bspF open reading frame missing the START and STOP codons was amplified from B. abortus genomic DNA using primers RC247 and RC248 (Reagents and Tools table) and cloned into the Eco RI and Bam HI restriction sites of pGBKT7 using the In‐Fusion ® PCR Cloning System (Clontech, 639650) to generate pGBKT7‐ bspF , which was verified by sequencing. pGBKT7‐ bspF was then transformed into the Y2HGold yeast strain using the Yeastmaker Yeast Transformation System 2 kit (Clontech). .. A Article Title: A Brucella effector modulates the Arf6‐Rab8a GTPase cascade to promote intravacuolar replication Article Snippet: Matchmaker Gold Yeast Two‐Hybrid system , Takara Bio USA, Inc , Cat#630489. .. Article Title: The Brucella abortus Type IV Effector BspA Inhibits MARCH6-Dependent ERAD To Promote Intracellular Growth. Article Snippet: Brucella abortus, the intracellular causative agent of brucellosis, relies on type IV secretion system (T4SS) effector-mediated modulation of host cell functions to establish a replicative niche, the Brucella-containing vacuole (BCV).. Brucella exploits the host’s endocytic, secretory, and autophagic pathways to modulate the nature and function of its vacuole from an endocytic BCV (eBCV) to an endoplasmic reticulum (ER)derived replicative BCV (rBCV) to an autophagic egress BCV (aBCV).. A role for the host ER-associated degradation pathway (ERAD) in the B. abortus intracellular cycle was recently uncovered, as it is enhanced by the T4SS effector BspL to control the timing of aBCV-mediated egress. Clone Assay:Article Title: A Brucella effector modulates the Arf6‐Rab8a GTPase cascade to promote intravacuolar replication Article Snippet: The bspF open reading frame missing the START and STOP codons was amplified from B. abortus genomic DNA using primers RC247 and RC248 (Reagents and Tools table) and cloned into the Eco RI and Bam HI restriction sites of pGBKT7 using the In‐Fusion ® PCR Cloning System (Clontech, 639650) to generate pGBKT7‐ bspF , which was verified by sequencing. pGBKT7‐ bspF was then transformed into the Y2HGold yeast strain using the Yeastmaker Yeast Transformation System 2 kit (Clontech). .. A Article Title: The Brucella abortus Type IV Effector BspA Inhibits MARCH6-Dependent ERAD To Promote Intracellular Growth. Article Snippet: Brucella abortus, the intracellular causative agent of brucellosis, relies on type IV secretion system (T4SS) effector-mediated modulation of host cell functions to establish a replicative niche, the Brucella-containing vacuole (BCV).. Brucella exploits the host’s endocytic, secretory, and autophagic pathways to modulate the nature and function of its vacuole from an endocytic BCV (eBCV) to an endoplasmic reticulum (ER)derived replicative BCV (rBCV) to an autophagic egress BCV (aBCV).. A role for the host ER-associated degradation pathway (ERAD) in the B. abortus intracellular cycle was recently uncovered, as it is enhanced by the T4SS effector BspL to control the timing of aBCV-mediated egress. Activation Assay:Article Title: A Brucella effector modulates the Arf6‐Rab8a GTPase cascade to promote intravacuolar replication Article Snippet: The bspF open reading frame missing the START and STOP codons was amplified from B. abortus genomic DNA using primers RC247 and RC248 (Reagents and Tools table) and cloned into the Eco RI and Bam HI restriction sites of pGBKT7 using the In‐Fusion ® PCR Cloning System (Clontech, 639650) to generate pGBKT7‐ bspF , which was verified by sequencing. pGBKT7‐ bspF was then transformed into the Y2HGold yeast strain using the Yeastmaker Yeast Transformation System 2 kit (Clontech). .. A Article Title: The Brucella abortus Type IV Effector BspA Inhibits MARCH6-Dependent ERAD To Promote Intracellular Growth. Article Snippet: Brucella abortus, the intracellular causative agent of brucellosis, relies on type IV secretion system (T4SS) effector-mediated modulation of host cell functions to establish a replicative niche, the Brucella-containing vacuole (BCV).. Brucella exploits the host’s endocytic, secretory, and autophagic pathways to modulate the nature and function of its vacuole from an endocytic BCV (eBCV) to an endoplasmic reticulum (ER)derived replicative BCV (rBCV) to an autophagic egress BCV (aBCV).. A role for the host ER-associated degradation pathway (ERAD) in the B. abortus intracellular cycle was recently uncovered, as it is enhanced by the T4SS effector BspL to control the timing of aBCV-mediated egress. Plasmid Preparation:Article Title: A Brucella effector modulates the Arf6‐Rab8a GTPase cascade to promote intravacuolar replication Article Snippet: The bspF open reading frame missing the START and STOP codons was amplified from B. abortus genomic DNA using primers RC247 and RC248 (Reagents and Tools table) and cloned into the Eco RI and Bam HI restriction sites of pGBKT7 using the In‐Fusion ® PCR Cloning System (Clontech, 639650) to generate pGBKT7‐ bspF , which was verified by sequencing. pGBKT7‐ bspF was then transformed into the Y2HGold yeast strain using the Yeastmaker Yeast Transformation System 2 kit (Clontech). .. A Transformation Assay:Article Title: A Brucella effector modulates the Arf6‐Rab8a GTPase cascade to promote intravacuolar replication Article Snippet: The bspF open reading frame missing the START and STOP codons was amplified from B. abortus genomic DNA using primers RC247 and RC248 (Reagents and Tools table) and cloned into the Eco RI and Bam HI restriction sites of pGBKT7 using the In‐Fusion ® PCR Cloning System (Clontech, 639650) to generate pGBKT7‐ bspF , which was verified by sequencing. pGBKT7‐ bspF was then transformed into the Y2HGold yeast strain using the Yeastmaker Yeast Transformation System 2 kit (Clontech). .. A Article Title: The Brucella abortus Type IV Effector BspA Inhibits MARCH6-Dependent ERAD To Promote Intracellular Growth. Article Snippet: Brucella abortus, the intracellular causative agent of brucellosis, relies on type IV secretion system (T4SS) effector-mediated modulation of host cell functions to establish a replicative niche, the Brucella-containing vacuole (BCV).. Brucella exploits the host’s endocytic, secretory, and autophagic pathways to modulate the nature and function of its vacuole from an endocytic BCV (eBCV) to an endoplasmic reticulum (ER)derived replicative BCV (rBCV) to an autophagic egress BCV (aBCV).. A role for the host ER-associated degradation pathway (ERAD) in the B. abortus intracellular cycle was recently uncovered, as it is enhanced by the T4SS effector BspL to control the timing of aBCV-mediated egress. |